| University of Texas Southwestern Medical Center | public educational institution of the United States, medical organization, medical school | — | 1943 |
| University of the People | nonprofit organization, community organization, private university | — | 2009 |
| University of the Virgin Islands | historically black college or university, land-grant university, public university | — | March 16, 1962 |
| University of Toledo | public educational institution of the United States, public research university | — | 1872 |
| University of Vermont | public educational institution of the United States, open-access publisher, land-grant university, public research university | Burlington | 1791 |
| University of Virginia School of Law | law school, faculty | — | 1819 |
| University of Wisconsin Press | university press | Madison | 1936 |
| University of Wisconsin System | state university system | Madison | 1848 |
| University of Wisconsin–La Crosse | public educational institution of the United States, public university | — | 1909 |
| University of Wisconsin–Madison | public educational institution of the United States, land-grant university, public research university | — | 1848 |
| University of Wisconsin–Milwaukee | research university, public educational institution of the United States, public university | Milwaukee | 1885 |
| University of Wyoming | public educational institution of the United States, land-grant university, public university | — | March 1886 |
| University System of Maryland | state university system | Baltimore | 1988 |
| UPA | film production company | Burbank | 1944 |
| Upjohn | pharmaceutical company, business, company | Michigan, Kalamazoo | 1886 |
| Upwork | organization, business, freelance marketplace, enterprise, public company | Palo Alto | 2015 |
| URS Corporation | business, enterprise | Transamerica Pyramid, San Francisco | 1951 |
| US Airways | airline | Tempe | 1937 |
| US Airways Express | airline | Tempe | 1967 |
| US Foods | business, public company | Rosemont | August 1, 1989 |
| USAA | insurance company, bank, business, financial institution | — | 1922 |
| Ushahidi Inc. | nonprofit organization, technology company | Nairobi | January 9, 2008 |
| UUNET | business | Ashburn | 1987 |
| Valero Energy | business, gas station chain, enterprise, public company | — | 1980 |
| Valiant Comics | comics publishing company, business | New York City | 1989 |
| Valparaiso University | private not-for-profit educational institution, open-access publisher, private university | Valparaiso | 1859 |
| Valve Corporation | computer manufacturer, software company, video game publisher, video game developer | Bellevue | 1996 |
| Vanguard Animation | production company, business | West Hollywood | 2002 |
| Varian Medical Systems | business, public company | Palo Alto | 1948 |
| VCA Pictures | pornographic film distributor, pornographic film studio | Culver City | — |
| Vector Motors | business, automobile manufacturer | — | 1978 |
| Vectrix | motorcycle manufacturer | Hong Kong | 2006 |
| Veeva Systems | business, public company | Pleasanton | 2007 |
| Venmo | online service, website, business | New York City | 2009 |
| Verbatim | business, enterprise | Charlotte | April 28, 1969 |
| Vercel Inc. | corporation, technology company, company | San Francisco | November 4, 2015 |
| VeriFone | business, public company | San Jose, Coral Springs | 1981 |
| Verso Books | publishing house | New York City, London | 1970 |
| Vertigo | book publisher, publishing house, imprint | Burbank, New York City | 1993 |
| VF Corporation | business, enterprise, public company | Denver, Greensboro | 1899 |
| Viacom | business, enterprise, public company | One Astor Plaza | December 31, 2005 |
| Viacom | production company, business | One Astor Plaza | May 3, 1971 |
| Viagogo | business, ticket sales service, ticket reseller | London | 2006 |
| Viatris | multinational corporation, pharmaceutical company, public company | Pittsburgh | November 2020 |
| Vici Properties | casino, real estate company, public company | New York City | October 6, 2017 |
| Victor Talking Machine Company | record company, business | Camden | 1901 |
| Victoria’s Secret | fashion label, business, brick and mortar, public company | Reynoldsburg | June 12, 1977 |
| Viki | online film database, website, business, online video platform, video streaming service, Internet audio-visual programming service provider | California | 2007 |