| Namesys | business | Oakland | 2004 |
| Naropa University | private not-for-profit educational institution, university | — | 1974 |
| NASA Space Science Data Coordinated Archive | data library | — | 1966 |
| National Academies of Sciences, Engineering, and Medicine | scientific organization, national academy, academy of sciences | Washington, D.C. | 1863 |
| National Academy of Engineering | academy of sciences | Washington, D.C. | 1964 |
| National Amusements | cinema chain | Norwood | 1936 |
| National Basketball Association | basketball association, professional sports league, basketball league, online database | — | August 3, 1949 |
| National Bureau of Economic Research | nonprofit organization, publishing house, research institute | Cambridge | 1920 |
| National Catholic Reporter | newspaper, publishing house | Kansas City | 1964 |
| National Defense University | national defense university, staff college | — | 1976 |
| National Fire Protection Association | production company, standards organization, nonprofit organization | Quincy | 1896 |
| National Football League | American football league, professional sports league | 345 Park Avenue | 1920 |
| National Gallery of Art | nonprofit organization, publishing house, United States federal agency, public–private partnership, national gallery | Washington, D.C. | March 24, 1937 |
| National Geographic Partners | publishing house, business, enterprise, company | New York City | September 9, 2015 |
| National Geographic Society | nonprofit organization, film production company, publishing house, scientific society | Washington, D.C. | 1888 |
| National Hockey League | professional sports league, ice hockey league | New York City | November 26, 1917 |
| National Instruments | business, enterprise, public company | Austin | 1976 |
| National Research Council | academy of sciences | — | June 19, 1916 |
| National War College | staff college, military school | Fort Lesley J. McNair | 1946 |
| National Wrestling Alliance | business, enterprise, professional wrestling promotion | Houston | 1948 |
| Nationwide Mutual Insurance Company | insurance company, business | One Nationwide Plaza | 1926 |
| Nature Portfolio | publishing house, open-access publisher, academic publisher | London | 1999 |
| Naughty America | pornographic film distributor, pornographic film studio | San Diego | 2001 |
| Naughty Dog | video game developer | Santa Monica | 1984 |
| Nautica | brand, business | New York City | 1983 |
| Naval History & Heritage Command | archives, command, museum, library | Washington Navy Yard | 1944 |
| Naval Postgraduate School | naval force university, staff college, public educational institution of the United States, open-access publisher | — | 1909 |
| NBC | radio network, production company, television network | NBC Tower, 10 Universal City Plaza, Stamford, 30 Rockefeller Plaza | 1939 |
| NBC News | production company, online newspaper, television channel, specialty channel | New York City, 30 Rockefeller Plaza | February 21, 1940 |
| NCR Corporation | business, enterprise, public company | Atlanta, Augsburg | 1884 |
| Neiman Marcus | business | Dallas | 1907 |
| NetApp | business, enterprise, public company | Sunnyvale | 1992 |
| Netflix, Inc. | television production company, film distributor, business, enterprise, public company | Los Gatos | August 29, 1997 |
| NetherRealm Studios | video game developer | Chicago | 2010 |
| NetJets | airline, business | Columbus | 1964 |
| Netlify | business | San Francisco | January 27, 2014 |
| NetSuite | business, enterprise, public company | Austin | 1998 |
| New Amsterdam Theatre | Broadway theatre, theatre building, movie theater | — | 1903 |
| New College of Florida | liberal arts college, Colleges That Change Lives, public educational institution of the United States | — | 1960 |
| New England Conservatory | conservatory, university building, private not-for-profit educational institution, college of music | — | 1867 |
| New Frontier Hotel and Casino | casino, destroyed building or structure, hotel, music venue | — | 1942 |
| New Mexico State University | public educational institution of the United States, open-access publisher, land-grant university, public university | University Park | 1888 |
| New World Computing | video game developer | Agoura Hills | 1984 |
| New World Pictures | film production company, holding company, film distributor | Atlanta | 1970 |
| New York Botanical Garden | nonprofit organization, botanical garden, educational institution | The Bronx, New York City | 1891 |
| New York Institute of Technology | private not-for-profit educational institution, university | — | 1955 |
| New York Mercantile Exchange | stock exchange, business | New York City | 1872 |
| New York University | research university, private not-for-profit educational institution, private university | New York City | 1831 |
| New York University Press | website, university press | New York City | 1916 |
| New York University School of Law | law school, faculty, organization | — | June 2, 1835 |
| Newegg | website, business | City of Industry | 2001 |
| Newgrounds | dot-com company, user-generated content platform, browser game website, online community, online art gallery | Glenside, Cheltenham Township | July 12, 1995 |
| Nickel City Opera | opera company | Buffalo | 2004 |
| Nickelodeon Animation Studio | animation studio | Studio City, Burbank, New York City | March 4, 1998 |
| Nightdive Studios | video game publisher, video game developer | Vancouver | 2013 |
| Nik Software | business | San Diego | 1995 |
| Nike | business, enterprise, public company | Beaverton | January 25, 1964 |
| Nintendo Software Technology | video game developer | Redmond | 1998 |
| Nomura Securities Co Ltd | stock exchange, business, enterprise | Nihonbashi, Tokyo | May 7, 2001 |
| Nordstrom | business, public company | Seattle | 1901 |
| Norfolk State University | historically black college or university, public educational institution of the United States, university | — | 1935 |
| North Carolina Central University | historically black college or university, public educational institution of the United States, university | Durham | 1909 |
| North Carolina State University | public educational institution of the United States, open-access publisher, land-grant university, public research university | Raleigh | 1887 |
| Northern Arizona University | public educational institution of the United States, open-access publisher, public research university | — | 1899 |
| Northern Illinois University | public educational institution of the United States, university, normal school | DeKalb | 1895 |
| Northern Kentucky University | public educational institution of the United States, public university | — | 1968 |
| Northrop Grumman Corporation | defense contractor, public company, aerospace manufacturer | Falls Church | 1994 |
| Northwestern University | private not-for-profit educational institution, research institute, private university | Evanston | 1851 |
| Norwegian Cruise Line | business, enterprise, public company, cruise line | Miami-Dade County | 1966 |
| NovaKid | privately held company, software, company | San Francisco | 2017 |
| NovaLogic | video game developer | Agoura Hills | 1985 |
| NStigate Games | video game developer | Marin County | March 1998 |
| Nu Skin Enterprises | multi-level marketing company, public company | Provo | 1984 |
| Nucor | business, enterprise, public company | Charlotte | September 1955 |
| NZXT | business | Los Angeles | 2004 |