| Edison Studios | film production company, film studio | United States | 1882 |
| Edition Peters | group of companies, music publisher | Frankfurt | December 1, 1800 |
| Einstein Bros. Bagels | restaurant chain, business, public company | Lakewood | 1995 |
| Elevance Health, Inc. | organization, business, enterprise, public company | Indianapolis | 2004 |
| Eli Lilly and Company | pharmaceutical company, organization, business, public company | Indianapolis | 1901 |
| Elon University | university | — | 1889 |
| Elsevier | book publisher, academic publisher | Amsterdam | 1880 |
| Embarcadero Technologies | business | Austin | October 1993 |
| Emerson College | private not-for-profit educational institution, university | Boston | 1880 |
| EMG, Inc. | business, musical instrument manufacturing company | Santa Rosa | 1976 |
| Energizer Holdings, Inc. | brand, business, enterprise, public company | St. Louis | 2000 |
| Enron | criminal organization, business, enterprise, public company | Houston | 1985 |
| Ensemble Studios | video game developer | Dallas | 1995 |
| Enstrom Helicopter Corporation | business | Menominee | 1959 |
| EnterpriseDB | business | Bedford | March 2004 |
| Envoy Air | airline | Fort Worth | 1984 |
| EPAM Systems | business, enterprise, public company | Newtown, Pennsylvania | 1993 |
| Epcot | theme park | — | October 1, 1982 |
| Epicor | business, enterprise | Austin, Irvine | 1972 |
| Epyx | video game publisher, video game developer, business, enterprise | San Francisco | 1978 |
| Equifax | credit bureau, business, public company | Atlanta | 1899 |
| Equinix | business, public company | Redwood City | June 22, 1998 |
| Esso | brand, gas station chain | Irving | 1911 |
| Estée Lauder Companies | business, enterprise, public company | Neuilly-sur-Seine, New York City | 1946 |
| Ethyl Corporation | organization, business, public company | Richmond | 1921 |
| Etsy | business, public company | Dumbo, New York, Brooklyn | June 18, 2005 |
| Euronet Worldwide | business, public company | Leawood | 1994 |
| Evergreen State College | liberal arts college, Colleges That Change Lives, public educational institution of the United States | — | 1967 |
| Everts Air | airline | Fairbanks | 1977 |
| Evolve | business | Philadelphia | 2009 |
| Excalibur Hotel and Casino | casino, building complex, hotel, resort | — | 1990 |
| Exelon | holding company, business, enterprise, public company | Chicago | 2000 |
| Exidy | business | Sunnyvale | 1974 |
| Expedia Group | business, enterprise, public company | Seattle | October 22, 1996 |
| Expedia.com | online travel agency, travel website, travel agency, mobile app | — | October 22, 1996 |
| Expeditors International | business, enterprise, public company | Seattle | 1979 |
| Export-Import Bank of the United States | corporation, independent agency of the United States government, state bank, state-owned enterprise, Export–Import Bank | Washington, D.C. | February 2, 1934 |
| ExpressJet | airline | Atlanta | 1986 |
| Extreme Championship Wrestling | media franchise, business | Philadelphia | April 25, 1992 |
| ExxonMobil | oil company, multinational corporation, business, gas station chain, enterprise, public company | Spring | August 5, 1882 |
| Eye-Fi | business, company | Mountain View | 2005 |
| F5, Inc. | software company, public company | Seattle | 1996 |
| Fairchild | aerospace manufacturer | — | 1925 |
| Fairfield University | private not-for-profit educational institution, Christian school, private university | — | 1942 |
| Fakku | pornography website, video game publisher, manga publisher | Portland | 2006 |
| Falcon Entertainment | pornographic film distributor, pornography website, pornographic film studio | San Francisco | 1971 |
| Fannie Mae | bank, business, state-owned enterprise of the United States | Northwest | 1938 |
| Fantagraphics Books | comics publishing company, book publisher | Seattle | 1976 |