| New World Pictures | film production company, holding company, film distributor | Atlanta | 1970 |
| New York Botanical Garden | nonprofit organization, botanical garden, educational institution | The Bronx, New York City | 1891 |
| New York Institute of Technology | private not-for-profit educational institution, university | — | 1955 |
| New York Mercantile Exchange | stock exchange, business | New York City | 1872 |
| New York University | research university, private not-for-profit educational institution, private university | New York City | 1831 |
| New York University Press | website, university press | New York City | 1916 |
| New York University School of Law | law school, faculty, organization | — | June 2, 1835 |
| Newegg | website, business | City of Industry | 2001 |
| Newgrounds | dot-com company, user-generated content platform, browser game website, online community, online art gallery | Glenside, Cheltenham Township | July 12, 1995 |
| Nickel City Opera | opera company | Buffalo | 2004 |
| Nickelodeon Animation Studio | animation studio | Studio City, Burbank, New York City | March 4, 1998 |
| Nightdive Studios | video game publisher, video game developer | Vancouver | 2013 |
| Nik Software | business | San Diego | 1995 |
| Nike | business, enterprise, public company | Beaverton | January 25, 1964 |
| Nintendo Software Technology | video game developer | Redmond | 1998 |
| Nomura Securities Co Ltd | stock exchange, business, enterprise | Nihonbashi, Tokyo | May 7, 2001 |
| Nordstrom | business, public company | Seattle | 1901 |
| Norfolk State University | historically black college or university, public educational institution of the United States, university | — | 1935 |
| North Carolina Central University | historically black college or university, public educational institution of the United States, university | Durham | 1909 |
| North Carolina State University | public educational institution of the United States, open-access publisher, land-grant university, public research university | Raleigh | 1887 |
| Northern Arizona University | public educational institution of the United States, open-access publisher, public research university | — | 1899 |
| Northern Illinois University | public educational institution of the United States, university, normal school | DeKalb | 1895 |
| Northern Kentucky University | public educational institution of the United States, public university | — | 1968 |
| Northrop Grumman Corporation | defense contractor, public company, aerospace manufacturer | Falls Church | 1994 |
| Northwestern University | private not-for-profit educational institution, research institute, private university | Evanston | 1851 |
| Norwegian Cruise Line | business, enterprise, public company, cruise line | Miami-Dade County | 1966 |
| NovaKid | privately held company, software, company | San Francisco | 2017 |
| NovaLogic | video game developer | Agoura Hills | 1985 |
| NStigate Games | video game developer | Marin County | March 1998 |
| Nu Skin Enterprises | multi-level marketing company, public company | Provo | 1984 |
| Nucor | business, enterprise, public company | Charlotte | September 1955 |
| NZXT | business | Los Angeles | 2004 |
| O'Reilly Auto Parts | business, public company | Springfield | 1957 |
| O.F. Mossberg & Sons | business, enterprise | North Haven | 1919 |
| Oakland Motor Car Company | automobile manufacturer | Pontiac | 1907 |
| Oberheim Electronics | business | Oakland | 1969 |
| Obsidian Entertainment | video game publisher, video game developer, business | Irvine | June 12, 2003 |
| Occidental Petroleum | business, public company | Houston | 1920 |
| Ocean Casino Resort | hotel | — | 2018 |
| Ocean Spray | agricultural cooperative, business | Lakeville | 1930 |
| Office Depot | business, retail chain, enterprise, public company | Boca Raton | 1986 |
| OfficeMax | business, retail chain | — | 1988 |
| Ogilvy | public relations agency, advertising agency | New York City | 1948 |
| Ohio University | public educational institution of the United States, public university | Athens | 1804 |
| Oikos University | university | Oakland | 2004 |
| Old Navy | business | San Francisco | 1994 |
| Oldsmobile | car brand, division, automobile manufacturer | Lansing | 1897 |
| Olin Corporation | business, enterprise, public company | Clayton | 1892 |