| University of Dayton | private not-for-profit educational institution, university | — | 1850 |
| University of Denver | research university, private not-for-profit educational institution, private university | Denver | 1864 |
| University of Evansville | private not-for-profit educational institution | Evansville | 1854 |
| University of Notre Dame | private not-for-profit educational institution, open-access publisher, Catholic university, private university, academic publisher | — | November 26, 1842 |
| University of Portland | private not-for-profit educational institution, Catholic university | — | 1901 |
| University of Puget Sound | liberal arts college, Colleges That Change Lives, private not-for-profit educational institution | Tacoma | 1888 |
| University of Richmond | private not-for-profit educational institution, university | Richmond | 1830 |
| University of Rochester | private not-for-profit educational institution, university | Rochester | 1850 |
| University of Saint Joseph | private not-for-profit educational institution, university | — | 1932 |
| University of San Diego | private not-for-profit educational institution, private university | San Diego | 1949 |
| University of St. Thomas | private not-for-profit educational institution, private university | Saint Paul | 1885 |
| University of Tampa | private not-for-profit educational institution, university | — | 1931 |
| Valparaiso University | private not-for-profit educational institution, open-access publisher, private university | Valparaiso | 1859 |
| Worcester Polytechnic Institute | private not-for-profit educational institution, university | — | 1865 |