| Eataly | Monticello d'Alba | 2010 |
| EBSCO Information Services | Birmingham | 1943 |
| Eckert–Mauchly Computer Corporation | Philadelphia | 1948 |
| Eckō Unltd. | East Brunswick | 1993 |
| Eclipse Comics | Forestville, Staten Island | 1977 |
| Ecolab | Saint Paul | 1923 |
| Einstein Bros. Bagels | Lakewood | 1995 |
| Elevance Health, Inc. | Indianapolis | 2004 |
| Eli Lilly and Company | Indianapolis | 1901 |
| Embarcadero Technologies | Austin | October 1993 |
| EMG, Inc. | Santa Rosa | 1976 |
| Energizer Holdings, Inc. | St. Louis | 2000 |
| Enron | Houston | 1985 |
| Enstrom Helicopter Corporation | Menominee | 1959 |
| EnterpriseDB | Bedford | March 2004 |
| EPAM Systems | Newtown, Pennsylvania | 1993 |
| Epicor | Austin, Irvine | 1972 |
| Epyx | San Francisco | 1978 |
| Equifax | Atlanta | 1899 |
| Equinix | Redwood City | June 22, 1998 |
| Estée Lauder Companies | Neuilly-sur-Seine, New York City | 1946 |
| Ethyl Corporation | Richmond | 1921 |
| Etsy | Dumbo, New York, Brooklyn | June 18, 2005 |
| Euronet Worldwide | Leawood | 1994 |
| Evolve | Philadelphia | 2009 |
| Exelon | Chicago | 2000 |
| Exidy | Sunnyvale | 1974 |
| Expedia Group | Seattle | October 22, 1996 |
| Expeditors International | Seattle | 1979 |
| Extreme Championship Wrestling | Philadelphia | April 25, 1992 |
| ExxonMobil | Spring | August 5, 1882 |
| Eye-Fi | Mountain View | 2005 |
| Fannie Mae | Northwest | 1938 |
| Fastenal | Winona | 1967 |
| Federal Home Loan Banks | Boston, Chicago, Pittsburgh, Dallas, Atlanta, Des Moines, Topeka, Cincinnati, New York City, San Francisco, Indianapolis | 1932 |
| FedEx Office | Dallas | 1970 |
| FEI Company | Hillsboro | 1971 |
| Fenway Sports Group | Boston | 2001 |
| Fidelity Investments | Boston | 1946 |
| Fidelity National Information Services | — | February 1, 2006 |
| Firebase | San Francisco | 2010 |
| Firefly Aerospace | Cedar Park | 2014 |
| Firehouse Subs | — | 1994 |
| First Data | Atlanta | 1969 |
| FirstEnergy | Akron | 1997 |
| Fiserv | Brookfield | 1984 |
| Fitbit | San Francisco | 2007 |
| Florsheim Shoes | Glendale | 1892 |